Cytology of body fluid pleural peritoneal pericardial ppt video. Test code cyasc cytology body fluid, ascitic specimen containers and fixatives. Vijay kumar bodal, preeti banasal, manjit singh bal, anil kumar suri, ranjeev bhagat, navneet kaur, mohanveer kaur and anikita goel. It is recommended that the initial assessment should concentrate on history, examination, and basic tests on ascitic fluid to assess the serumascites albumin gradient.
Pericardial fluid from the sac that surrounds the heart ascitic fluid, also called ascites or peritoneal fluid from the space in the belly scrape or brush cytology. Ascitic fluids exist to lubricate the organs and ensure. The authors have provided a general picture of the field, the details of which may be filled in by reference to the many fine sources available. To determine the presence of infection in the ascitic fluid c.
Ascitic fluid is a fluid that is also called peritoneal cavity fluid. Primary diagnosis of lymphomas from ascitic fluid is rare. Get 1550% off on diagnostic tests at top diagnostic centers. A simplified description of ascitic fluid analysis. Ascitic fluid analysis in hepatocellular carcinoma. In an attempt to identify and profile the population of ascites cells obtained from ovarian cancer patients, a novel method was developed to separate adherent ad and nonadherent nad cells in culture. Peritoneal tuberculosis as a cause of ascites in a patient. If abdominocentesis is indicated, but is negative after a complete fourquadrant paracentesis has been performed, diagnostic. Diagnostic algorithm for determining primary tumor sites using.
Specimen collection and processing instructions for medical laboratory test peritoneal fluid at geisinger medical laboratories. Chapter 4 diagnostic pitfalls in effusion fluid cytology vinod b. A peritoneal fluid culture is a test that is performed on a small sample of peritoneal fluid. Pleural fluid or ascites cytology specimen preparation. Analysis of ascitic fluid for cytological and biochemical findings. Normally contains very little fluid effusion develops from imbalance of fluid formation and removal. Tumors of borderline malignancy were included in the series. Paracentesis is a procedure in which a needle or catheter is inserted into the peritoneal cavity to obtain ascitic fluid for diagnostic or therapeutic purposes. Top tips for ascitic tap paracentesis always send the most fluid to cytology, especially if malignancy is suspected. Cytology of ascitic fluid and prostate acid phosphatase pap test. Analysis of ascetic fluid for cytological and biochemical findingsx. Cytology of body fluid free download as powerpoint presentation.
Fluid analysis fluid analysis begins with appropriate sample collection. Variation in the microscopic impression obtained by cytospin smears and cell block methods in. Cytologic examination of ascitic fluid in a patient with pleuralbased mass. If you want to gain a larger sample use a 50ml syringe to aspirate fluid. Advanced health practitioner must perform this procedure at least three times per year. Ascitic fluid for cytology prices starting at rs 600 in. Abnormal results are an indication for further nonroutine tests on another specimen.
Although fluid cytology poses diagnostic dilemma in few cases, still it is cost. This approach, normally performed by a junior member of the team could, in many cases, be. Cytology in peritoneal carcinomatosis sensitivity increased by centrifuging large. This monograph essentials of fluid cytology is written for practicing. A relatively simple bedside procedure in which one inserts a needle into the abdomen, thereby evacuating either a small amount of ascites fluid for diagnostic purposes, or large amounts of fluid for therapeutic purposes. Fluid analysis cytology was positive for adenocarcinoma.
If you continue browsing the site, you agree to the use of cookies on this website. Each candidate will be initially proctored and signed off by an attending physician. The fresh fluid was sent to the cytology laboratory where filter preparations, smears, and cell blocks were prepared by standard methods. Download scientific diagram cytology of ascitic fluid and prostate acid. Another cytology technique is to gently scrape or brush some cells from the organ or tissue being tested. Pleural effusion showing monomorphic population of atypical cells in a case of. Application of cytokeratin 7 and 20 immunohistochemistry. Department of pathology, government medical college, patila, punjab, india. In cases where this minimum is not met, the attending, must again sign off the procedure for the advanced health practitioner. Patients with cirrhosis develop ascites for physiologic reasons that are unrelated to malignant progression. How valuable is ascitic cytology in the detection and. In five of the false negative cases the initial investigation after presentation was an ascitic fluid aspiration. Worlds best powerpoint templates crystalgraphics offers more powerpoint templates than anyone else in the world, with over 4 million to choose from. We decided to proceed with transplantation as there was no sign of active bacterial peritonitis.
This book is concerned with a discussion of the basic facts, concepts, and problems of cellular biology. Marilia arcadipane, luis alberto lage and juliana pereira. Isolation and characterization of tumor cells from the. Diagnostic utility of cell block method versus cytospin. This may result from either a traumatic tap or malignancy. The more fluid sent, the higher the diagnostic yield. Peritoneal paracentesis adult, peds uc san diego health.
Ppt cytology of body fluid powerpoint presentation, free. Analysis of ascitic fluid for cytological and biochemical. How valuable is ascitic cytology in the detection and management of. Mean age of presentation in non malignant cases was 46. The bestknown cytology test that samples cells this way is the pap test. Ascitic fluid what do you do muhammad asim rana mbbs, mrcp, mrcps, fccp, edic, sfccm critical care medicine king saud medical city 2. View and download powerpoint presentations on body fluid cytology ppt. This video provides instruction on the proper way to aliquot and transport pleural fluid or ascites cytology specimens to a pathology. Most ascitic fluid is transparent and tinged yellow. Analysis of ascetic fluid for cytological and biochemical. Work up laboratory studies in patients with newonset ascites of unknown origin, peritoneal fluid should be sent for. Ascitic fluid glucose, ldh and total proteins may be helpful in ddx.
Cytokeratins 7 and 20 are differentially expressed by different normal tissues. A minimum of 10,000 red blood cellsl is required for ascitic fluid to appear pink. Abdominal paracentesis is a bedside procedure where the ascitic fluid is collected by insertion of needle into the peritoneal cavity. Winner of the standing ovation award for best powerpoint templates from presentations magazine. Cytology and fluid analysis of the acute abdomen tesis can be helpful in making a definitive diagnosis more rap idly. If you continue browsing the site, you agree to the use of. Although the histologic attributes of pmp have been well studied, the cytologic features remain. Pdf cytomorphological assessment of different body fluids. Aim of the presentation is to give a very clear understanding about the analysis of ascities. Cytology showed benign mesothelial cells and mixed inflammatory cells. Ascitic fluid cytomorphologic useless findings cytoplasmic vacuoles signet ring cells individual psammoma bodies cell within cell.
Ascitic fluid may be used to help determine the etiology of ascites, as well as to evaluate for infection or presence of cancer. Ascitic fluid cytology numerous pleomorphic lymphoid cells in primary effusion lymphoma. Ascitic fluid cytology numerous pleomorphic lymphoid. However, physicians performing paracentesis in these patients, often send fluid to the cytology laboratory, sometimes specifically looking for hepatocellular carcinoma hcc. A male lifelong nonsmoker aged 58 years with no prior asbestos exposure complained of gradual worsening breathlessness over 3 months. Book an appointment at a high quality center in hyderabad. Presentation will help the medical residents diagnose the cause of fluid accumulation in abdomen and thus will. Label the container with patients name, hospital number, room number, date, and type of specimen. Pdf ascitic fluid analysis in hepatocellular carcinoma. Peritoneal fluid cytology in patients with ovarian cancer. Ppt cytology powerpoint presentation free to view id.
Culture of the ascitic fluid was negative for bacteria and fungi. Fluid was obtained by aspiration of ascitic fluid 74 patients or by peritoneal washings at the time oflaparotomy 20 patients. The article deals with cytopathology specimens from spaces lined with mesothelium, i. Free cytology books download ebooks online textbooks.
Specimen collection and processing instructions for. Theyll give your presentations a professional, memorable appearance the kind of sophisticated look that todays audiences expect. It may also be called an abdominal tap or paracentesis. Find powerpoint presentations and slides using the power of, find free presentations research about body fluid cytology ppt. Shidham table of contents factors leading to potential diagnostic pitfalls 43 surfacetensionrelated alterations in cytomorphology 43 improper specimen. Ascitic fluid ascites is the abnormal accumulation of serous fluid in the peritoneal abdominal cavity and the fluid thus accumulated is referred to as ascetic fluid. Patient was discharged on synthroid 112 mcg daily, protonix 40. This is a fluid that is normally a part of an area of the body called the peritoneum, which consists of the digestive cavity. Too much hope is placed on ascitic cytology to provide the diagnosis at the expense of other investigations. Cytologic examination of ascitic fluid in a patient with pleuralbased.
We have investigated the diagnostic yield of these specimens. Ppt ascites powerpoint presentation free to download. Pseudomyxoma peritonei pmp is a rare condition characterized by gelatinous ascites. Tumor cells in ascites are a major source of disease recurrence in ovarian cancer patients. Nonspecific finding in women in ascitic fluids and pelvic washings. Never dispose of unused fluid, put it in an extra pot and add to the cytology sample. Many tumours retain the immunophenotype of the tissue from which they are derived and hence cytokeratin 7 and 20 immunostaining has potential value in diagnostic pathology. As regards cytological examination for malignant cells in ascitic fluid, positive cytology was recorded. Under ultrasound guidance the patient underwent paracentesis for removal of 3,500 ml of thin, nonpurulent, ascitic fluid. Effusion cytology is the study of individual cells. Ascites is abnormal excessive accumulation of fluid in peritoneal cavity it is the most common of the 3 major complications of cirrhosis ascites, hepatic encephalopathy and bleeding varices hepatology 1987.